ABSTRACT
Topiramate is an anticonvulsant used to treat seizures and prevent migraines. The aim of this study was to develop andvalidate a simple and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to quantifyTopiramate in its formulation. Acetonitrile and ammonium acetate were used as a mobile phase (85:15 v/v ratio),and isocratic elution mode was used for separation using Zorbax RP-C18Column (50 mm × 4.6 mm i.d., 5 µ) asa stationary phase. The standard calibration curve ranges from 1 to 1,000 ng/ml with a correlation coefficient of0.9990 (R2). The detection and quantification limits were obtained at 0.5 and 1.0 ng/ml, respectively. The total runtimeof chromatographic separation was found to be 2.0 minutes with a retention time of 1.23 minutes. The percentagerecovery studies were found to be 90.3%–99.3%. The developed method was found to be simple and sensitive and canbe used for the estimation of Topiramate in bulk and its pharmaceutical formulations.
ABSTRACT
Selegiline HCl is an irreversible MAO-B inhibitor used to reduce symptoms in early-stage Parkinson’s disease. It isused as an adjunct to drugs, such as L Dopamine (L-DOPA). The present study is designed to develop and validatea rapid, sensitive, and straightforward separation method with Electrospray ionization and triple quadrupole massanalyzer for the quantification of Selegiline HCl in bulk and pharmaceutical formulation. Zorbax C18 column (50 mm× 4.6 mm i.d, 5 µ particle size) was used for the separation of analyte and internal standard. The samples were elutedusing 0.1% Formic acid in water and Methanol (40:60%v/v) which is delivered at 0.5 ml/minute flow rate, with achromatographic runtime of 5 minutes. The eluents were monitored using a tandem mass spectrometer equipped withan electrospray ionization in positive mode and a triple quadrupole mass analyzer. The detection was carried out inmultiple reaction-monitoring mode by quantifying the m/z 188.05→91.10 ion transition pair; with collision energy−29.0 V for Selegiline HCl. Linearity was achieved over the concentration range 3.5–6.5 ng/ml for the developedmethod. The limit of detection and limit of quantification were found to be 0.2 and 0.5 ng/ml, respectively. Thecorrelation coefficient (r2) value was ≥0.9985 for Selegiline HCl. This method offers a sensitive quantification ofSelegiline HCl in the pharmaceutical formulation.